Effect of fractalkine on the expression and secretion of MMP-2 in human monocytes

AIM: To study the effect of fractalkine (CX3CL1, Fkn) on the expression and secretion of matrix metalloproteinase-2 (MMP-2) in cultured human monocyte line U937 cells. METHODS: The cultured U937 cells were incubated with recombinant human Fkn, the supernatant of human renal mesangial cells (HRMC) and Fkn neutralizing antibodies for 24 h. The mRNA expression of MMP-2 was analyzed by RT-PCR. The production of MMP-2 in the supernatant was analyzed by gelatin zymography. RESULTS: The level of MMP-2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group. Similarly, the level of MMP-2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group. However, the level of MMP-2 mRNA and protein in the cells incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC. CONCLUSION: Fkn inhibits the expression and secretion of MMP-2 in cultured U937 cells. HRMC might mediate the expression and secretion of MMP-2 in U937 cells through Fkn.     

黑龙江省农机购置补贴工作实施成效及建议

介绍近几年黑龙江省农机购置补贴工作的实施措施和成效，指出购机补贴工作中存在的问题．并对今后的农机购置补贴工作提出几点建议，以促进全省农机结构更加趋向合理化，推动农业机械化水平进一步提高，实现粮食增产、农业增效、农民增收。     

农村变电站选址规划方法

农村变电站选址是电网规划的重要组成部分,在农网规划是起承上启下的作用,文中介绍最优化选址问题,并将优化选址的数学模型进行分类,又全面地比较分析应用于变电站选址方法的诸多算法,提出5项有利于电力市场环境下的农网变电站选址的建议.     

杉木新造幼林施肥方案优选的初步研究

杉木新造幼林不同施肥方案的试验结果表明,施肥对杉木新造幼树生长具有明显的促进作用。经多目标决策分析确定,造林前施基肥钙镁磷600 kg.hm-2、造林当年追肥施复合肥180 kg.hm-2为最佳施肥方案,其次为施基肥钙镁磷600 kg.hm-2、追肥施尿素180 kg.hm-2。生产上可根据实际情况选择应用。     

Collection,Evaluation,and Utilization of Cotton Germplasm in China

A total of 8193 accessions,including 6822 Gossypium hirsutum,350 G.hirsutum race (sub-spe-cies),385 of G.barbadense,378 of G.arboreum,17 of G.herbaceum and 41 wild species,of cotton germplasm are now maintained in China.This germplasm is kept in Beijing National Long-term Genebank and Anyang Cotton Medium-term Genebank.Live plants of the wild species are kept in Cotton garden at Hainan Island.     

哲罗鱼精子的超微结构

应用透射电镜观察了乌苏里江哲罗鱼(Hucho taimen)精子的超微结构.哲罗鱼的精子由头部、中段和尾部组成.头部呈卵圆形,主要结构是细胞核.核前端无顶体,后端有植入窝,核中染色质致密,存在着不规则的网络状间隙.中段包括中心粒复合体和袖套.近端中心粒为9组三联微管结构,与远端中心粒相互垂直.袖套与细胞核后端相连,含有丰富的线粒体和囊泡,部分线粒体彼此融合,形成复合线粒体.尾部细长,主要结构是轴丝,为典型的"9 2"微管结构.尾部的近核段有许多囊泡包围着轴丝,远核段则无此结构.尾部有对称排列的波纹状侧鳍.     

坚持以人为本 优化教师管理

“以教师为本”是“以人为本”的体现，是学校发展的关键。实行“以人为本”的教师管理，要树立人才强校的办学理念，全面提高教师素质，改变绩效管理模式，为教师创造良好的学习、生活环境。     

论会计的诉讼职能及生成原因

在(ADA)框架下,会计不仅具有传统的核算职能和监督职能,而且还具有诉讼职能.会计的诉讼职能是由包括ADA在内的各国反倾销法直接赋予的.     

基于LMIs的不确定随机切换系统的H∞鲁棒控制

基于线性矩阵不等式和控制理论,针对由一组由随机微分方程描述的子系统组成的切换系统,研究此类切换系统在任意切换策略下的鲁棒控制问题,并给出控制器的设计方法.仿真结果表明,所设计的控制器能保证闭环系统在一定意义下的随机稳定.